Neutral Red |
| Catalog No.GC61835 |
Neutral Red, a nitrogenous pH-indicator with a pKi of 6.8, is an indicator for the internal acidification of thylakoids.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 553-24-2
Sample solution is provided at 25 µL, 10mM.
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Neutral Red, a nitrogenous pH-indicator with a pKi of 6.8, is an indicator for the internal acidification of thylakoids. Neutral Red stains lysosomes red[1].
As cells begin to die, their ability to incorporate neutral red diminishes. The neutral red is also used to stain cell cultures for plate titration of viruses[2].
References:
[1]. Winfried AUSLANDER, et al. NEUTRAL RED, A RAPID INDICATOR FOR pH-CHANGES IN THE INNER PHASE OF THYLAKOIDS. FEBS LETTERS. 1975 Nov.
[2]. Ellen Borenfreund, et al. A simple quantitative procedure using monolayer cultures for cytotoxicity assays (HTD/NR-90). Journal of tissue culture methods volume 9, pages7-9 (1985).
| Cas No. | 553-24-2 | SDF | |
| Canonical SMILES | NC1=CC2=NC3=CC(N(C)C)=CC=C3N=C2C=C1C.[H]Cl | ||
| Formula | C15H17ClN4 | M.Wt | 288.78 |
| Solubility | DMSO : 50 mg/mL (173.14 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.4628 mL | 17.3142 mL | 34.6284 mL |
| 5 mM | 0.6926 mL | 3.4628 mL | 6.9257 mL |
| 10 mM | 0.3463 mL | 1.7314 mL | 3.4628 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 40 reference(s) in Google Scholar.)
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