Nicotinamide riboside-d4 (triflate) |
| Catalog No.GC52214 |
An internal standard for the quantification of nicotinamide riboside
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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Nicotinamide riboside-d4 is intended for use as an internal standard for the quantification of nicotinamide riboside by GC- or LC-MS. Nicotinamide riboside is a riboside form of nicotinamide that is found in trace amounts in yeast-containing and milk-derived products.1 It is a precursor of NAD+ and a source of vitamin B3 (niacin). Nicotinamide riboside increases intracellular and mitochondrial NAD+ content in C2C12, Hepa1.6, and HEK293 cells when used at concentrations ranging from 1-1,000 μM.2 It also decreases acetylation of FOXO1 and SOD2, which are substrates of sirtuin 1 (SIRT1) and SIRT3, respectively, but not the SIRT2 substrate tubulin, indicating nicotinamide riboside selectively enhances SIRT1 and SIRT3 deacetylase activity. Nicotinamide riboside (400 mg/kg per day) increases NAD+ levels in liver and skeletal muscle and prevents body weight gain in mice fed a high-fat diet. It also increases NAD+ in the cerebral cortex and reduces cognitive deterioration in a transgenic mouse model of Alzheimer's disease.3
1.Chi, Y., and Sauve, A.A.Nicotinamide riboside, a trace nutrient in foods, is a vitamin B3 with effects on energy metabolism and neuroprotectionCurr. Opin. Clin. Nutr. Metab. Care15(6)657-661(2013) 2.CantÓ, C., Houtkooper, R.H., Pirinen, E., et al.The NAD+ precursor nicotinamide riboside enhances oxidative metabolism and protects against high-fat diet-induced obesityCell Metab.15(6)838-847(2012) 3.Gong, B., Pan, Y., Vempati, P., et al.Nicotinamide riboside restores cognition through an upregulation of proliferator-activated receptor-γ coactivator 1α regulated β-secretase 1 degradation and mitochondrial gene expression in Alzheimer's mouse modelsNeurobiol. Aging34(6)1581-1588(2013)
| Cas No. | SDF | ||
| Canonical SMILES | O[C@H]1[C@@]([N+]2=C([2H])C([2H])=C([2H])C(C(N)=O)=C2[2H])([H])O[C@@H]([C@H]1O)CO.FC(F)(S(=O)([O-])=O)F | ||
| Formula | C11H11D4N2O5 ? CF3SO3 | M.Wt | 408.3 |
| Solubility | Methanol: soluble,Water: soluble | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.4492 mL | 12.2459 mL | 24.4918 mL |
| 5 mM | 0.4898 mL | 2.4492 mL | 4.8984 mL |
| 10 mM | 0.2449 mL | 1.2246 mL | 2.4492 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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