Nicotine (Synonyms: DL-Nicotine) |
| Catalog No.GC14043 |
A nicotinic acetylcholine receptor (nAChR) agonist
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 22083-74-5
Sample solution is provided at 25 µL, 10mM.
(±)-Nicotine is the racemic mixture of the dominant alkaloid found in tobacco plants. It acts as an agonist at neuronal nicotinic acetylcholine receptors (nAChRs; Kis = 481 and 11.1 nM for α3β4 and α4β2 subtypes, respectively) and possesses addictive and teratogenic properties.1 (−)-(S)-Nicotine is significantly more active at binding nAChRs compared to the (+)-(R) antipode, thus nicotine is typically synthesized as (−)-(S)-nicotine with only 0.2-1% of the (+)-(R) isomer present.2
References
1. Zaveri, N., Jiang, F., Olsen, C., et al. Novel α3β4 nicotinic acetylcholine receptor-selective ligands. Discovery, structure-activity studies, and pharmacological evaluation. J. Med. Chem. 53(22), 8187-8191 (2010).
2. Clayton, P., Lu, A., and Bishop, L. The pyrolysis of (−)-(S)-nicotine: Racemization and decomposition. Chirality 22(4), 442-446 (2010).
| Cas No. | 22083-74-5 | SDF | |
| Synonyms | DL-Nicotine | ||
| Chemical Name | (R)-3-(1-methylpyrrolidin-2-yl)pyridine | ||
| Canonical SMILES | CN1[C@@H](C2=CC=CN=C2)CCC1 | ||
| Formula | C10H14N2 | M.Wt | 162.23 |
| Solubility | 30mg/mL in DMSO, 50mg/mL in DMF, 50mg/mL in Ethanol | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 6.1641 mL | 30.8204 mL | 61.6409 mL |
| 5 mM | 1.2328 mL | 6.1641 mL | 12.3282 mL |
| 10 mM | 0.6164 mL | 3.082 mL | 6.1641 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Related Biological Data
Nicotine activates the prok2R/Akt/FoxO3a axis and exhibits anti-apoptotic effects against MPP+in the HEK293T cell line.(e) and semi-quantitative analysis of fluorescence intensity
Nicotine (GLPBIO) was dissolved in DMSO to form the concentration of 20 mg/100 μL. Then the solution was diluted to 10 mL using saline to 2 mg/mL.
J Transl Med 22.1 (2024): 1-21. PMID: 38609979 IF: 7.4001 -
Related Biological Data
The role of Met in hyperlipidaemia-associated vascular calcification.(A) Experimental design: SD rats in four groups were subjected to the indicated treatment for 6 weeks in total. (B) Representative Von Kossa and H&E staining in aortic tissues of SD rats (scale bars = 500 μm).
To establish a vascular calcification model, rats were injected with vitamin D3 (300,000 IU/kg) and simultaneously intragastrically administered nicotine (25 mg/kg in 3 mL peanut oil) (GLPBIO)at 9a.m. on the first day.
Free Radical Biology and Medicine (2021). PMID: 33513420 IF: 7.373 -
Related Biological Data
OEA alleviates vascular calcification caused by hyperlipidemia in vivo and PA in vitro. (A) Schematic protocol of animal feed and treatment. (B, C)Representative Von Kossa (B) and H&E (C) staining on thoracic aorta slices of SD rats (Scale bars: 500 μm, n = 6–8).
Nicotine (GLPBIO)(25 mg/kg/day in 3 mL peanut oil) was injected, gavaged, respectively, and nicotine intervention was repeated after 8 h on the same day for inducing the model of vascular calcification.
Biochemical Pharmacology 208 (2023): 115379. PMID: 36525991 IF: 6.1004
Average Rating: 5 (Based on Reviews and 33 reference(s) in Google Scholar.)
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