Ac-LEHD-AMC (trifluoroacetate salt) (Synonyms: Ac-Leu-Glu-His-Asp-AMC, Caspase-9 Substrate) |
| Catalog No.GC18226 |
Ac-LEHD-AMC (trifluoroacetate salt) is a fluorogenic substrate for caspase-9 (Excitation: 341 nm; Emission: 441 nm).
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Cas No.: 292633-16-0
Sample solution is provided at 25 µL, 10mM.
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Ac-LEHD-AMC is a fluorogenic substrate for caspase-9.[1] Upon cleavage by caspase-9, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify caspase-9 activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.
References
[1]. Prudnikov, I.M., and Smirnov, A.N. Short peptide tools for monitoring caspase and proteasome activities in embryonal and adult rat brain lysates: An approach for the differential identification of proteases. J. Biochem. 151(3), 299-316 (2012).
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