STY-BODIPY (Synonyms: Styrene-BODIPY)

This plan only provides a guide, please modify it to meet your specific needs.
STY-BODIPY is a highly absorbable auto-oxidizable co-substrate, and the reaction progress can be monitored by detecting the consumption of STY-BODIPY.
Application example: STY-BODIPY serves as a signal carrier for inhibited auto-oxidation and can determine the inhibition rate constant (kinh) and stoichiometry (n) of the reaction between the inhibitor and chain-carrying peroxyl radicals.
Literature source:
Ron Shah,Mikhail S Shchepinov,Derek A Pratt. Resolving the Role of Lipoxygenases in the Initiation and Execution of Ferroptosis. ACS Cent Sci. 2018 Mar 28;4(3):387-396. doi: 10.1021/acscentsci.7b00589. Epub 2018 Feb 7.
experimental method:
1. Add liposomes (125 µL of 20mM PBS solution, pH 7.4) to a cuvette containing 2.34 mL of PBS (pH 7.4, 10 mM) and equilibrate the solution for 5 min at 37°C (as described from Egg -PC forms liposomes);
2. Empty the cuvette, add 10 µL of 2 mM STY-BODIPY in DMSO, then add 10 µL of 0.05M MeOAMVN in acetonitrile, and mix the solution thoroughly;
3. After 5 minutes, add an aliquot of the LOX inhibitor or RTA stock solution (1 mM in DMSO) and measure the absorbance loss at 565 nm to determine the inhibition rate constant (kinh) and stoichiometry (n) for each experiment according to the equation ). They are (1) and (2) respectively. Kinetic data are given as the average of three independent measurements. Indistinguishable results were obtained in selected control experiments in which antioxidants were added before liposome removal.
Example of experimental results:

STY-BODIPY (A) serves as the signal carrier in inhibited autoxidations, enabling determination of inhibition rate constants (kinh) and stoichiometries (n) for reactions of inhibitors with chain-carrying peroxyl radicals (eqs 1 and 2, respectively). Coautoxidations of cumene (3.6 M) and STY-BODIPY (10 μM) initiated by AIBN (6 mM) in chlorobenzene at 37 °C (black) and inhibited by 2 μM of NDGA, zileuton, PD146176, CAY10649, CJ-13610, Lip-1, Fer-1, C15-THN, α-TOH, and PMC (B). Coautoxidations of egg phosphatidylcholine liposomes (1.0 mM) and STY-BODIPY (10 μM) suspended in phosphate-buffered saline (10 mM) at pH 7.4 initiated by MeOAMVN (0.2 mM) at 37 °C (black) and inhibited by 2 μM of NDGA, zileuton, PD146176, CAY10649, CJ-13610, Lip-1, Fer-1, C15-THN, α-TOH, and PMC (C). Inhibition rate constants obtained from coautoxidations of cumene (D) or phosphatidylcholine liposomes (E) with STY-BODIPY at 37 °C.

Precautions:
1) Fluorescent dyes all have quenching problems. Please avoid light as much as possible to slow down fluorescence quenching.
2) For your safety and health, please wear a lab coat and disposable gloves.