Pseudothymidine (5-Methyl-2'-Deoxypseudouridin) |
Catalog No.GC33944 |
Pseudothymidine (5-Methyl-2'-Deoxypseudouridin) is a C-nucleoside analog of thymidine.
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Cas No.: 65358-15-8
Sample solution is provided at 25 µL, 10mM.
Pseudothymidine is a C-nucleoside analog of thymidine.
Pseudothymidine is a C-nucleoside analog of thymidine[1]. The calculated δδG°50/mod is -0.5 kcal/mol, with a δTm/mod of 0.82°C. For the duplexes containing nine dA-T/ψT pairs, the δTm/mod is -0.9°C and a δδG°50/mod is +1.1 kcal/mol. The modification of the duplex containing 12 consecutive dA-T/ψT base pairs produces a δTm/mod of -0.9°C and a δδG°50/mod of +1.2 kcal/mol[2].
[1]. S Lutz, et al. An in vitro screening technique for DNA polymerases that can incorporate modified nucleotides. Pseudo-thymidine as a substrate for thermostable polymerases. Nucleic Acids Res. 1999 Jul 1; 27(13): 2792-2798. [2]. Havemann SA, et al. Incorporation of multiple sequential pseudothymidines by DNA polymerases and their impact on DNA duplex structure. Nucleosides Nucleotides Nucleic Acids. 2008 Mar;27(3):261-78.
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