Rosmarinic acid |
| Catalog No.GN10648 |
Rosmarinic acid is a widespread phenolic ester compound in the plants. Rosmarinic acid has antiviral, antibacterial, antiinflammatory, neuroprotective, anticancer, anti-lipid peroxidative, apoptotic,and antioxidant activities.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 20283-92-5
Sample solution is provided at 25 µL, 10mM.
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Rosmarinic acid is a widespread phenolic ester compound in the plants. Rosmarinic acid has antiviral, antibacterial, antiinflammatory, neuroprotective, anticancer, anti-lipid peroxidative, apoptotic,and antioxidant activities[1]. Rosmarinic acid has the ability to block complement fixation, inhibit lipoxygenase and cyclooxygenase activity and inhibit the expression of CCL11 and CCR3 by suppressing the IKK-β activity in NF-κB activation signaling[2].
Rosmarinic Acid (2, 4µg/mL; 24h) Promote NHE1 Expression In Vitro[3].Rosmarinic acid (10-100µg/mL; 12h) inhibited ROS and MMP-1 production in NHDF cells induced by H2O2 and UVA, respectively, without exerted cytotoxicity; and Nitric oxide and inducible nitric oxide synthase (iNOS) productions were inhibited by Rosmarinic acid (10-100µg/mL; 12h) in RAW264.7 mouse macrophage cells induced by combined LPS-IFN-γ[4].
Rosmarinic acid (50, 100mg/kg; po, 8 weeks) mediates reverse cholesterol transport through increased plasma cholesterol uptake and subsequent hepatobiliary excretion and induces lipid metabolism by increasing fatty acid β-oxidation in AMPK-dependent activation of CPT1A, thus modulating lipid accumulation and attenuating hyperlipidemia in HFD-fed mice[5].Rosmarinic acid (100mg/kg; po; 30d) alleviates pancreatic β-cell dysfunction and glucolipotoxicity-mediated oxidative stress during HFD-STZ-induced type 2 diabetes mellitus, perhaps through its antioxidant potential[6].
References:
[1].El Kantar S, Yassin A, Nehmeh B, et al. Deciphering the therapeutical potentials of rosmarinic acid[J]. Scientific Reports, 2022, 12(1): 15489.
[2].Lee J, Jung E, Kim Y, et al. Rosmarinic acid as a downstream inhibitor of IKK‐β in TNF‐α‐induced upregulation of CCL11 and CCR3[J]. British journal of pharmacology, 2006, 148(3): 366-375.
[3]. Jung S W, Park G H, Kim E, et al. Rosmarinic acid, as an NHE1 activator, decreases skin surface pH and improves the skin barrier function[J]. International journal of molecular sciences, 2022, 23(7): 3910.
[4].Pattananandecha T, Apichai S, Julsrigival J, et al. Antioxidant activity and anti-photoaging effects on UVA-irradiated human fibroblasts of rosmarinic acid enriched extract prepared from Thunbergia laurifolia leaves[J]. Plants, 2021, 10(8): 1648.
[5]. Nyandwi J B, Ko Y S, Jin H, et al. Rosmarinic acid exhibits a lipid-lowering effect by modulating the expression of reverse cholesterol transporters and lipid metabolism in high-fat diet-fed mice[J]. Biomolecules, 2021, 11(10): 1470.
[6].Govindaraj J, Sorimuthu Pillai S. Rosmarinic acid modulates the antioxidant status and protects pancreatic tissues from glucolipotoxicity mediated oxidative stress in high-fat diet: streptozotocin-induced diabetic rats[J]. Molecular and cellular biochemistry, 2015, 404: 143-159.
| Cell experiment [1]: | |
Cell lines | Normal human epidermal keratinocytes cells |
Preparation Method | Rosmarinic acid was dissolved in dimethyl sulfoxide (DMSO) and mixed in KBM medium at concentrations of 2µg/mL and 4µg/mL.Normal human epidermal keratinocytes (NHEKs) were cultured in keratinocyte growth medium (KGM) in a 75cm2 flask. Cells were collected by trypsin treatment and diluted with KGM to obtain a final density of 1.5×105 cells/mL; then, 2mL of the cell mixture was inoculated onto a 35mm diameter dish and cultured overnight. KGM was then replaced with KBM, and the cells were cultured for 24h. A test sample was dissolved in KBM and added to the dish. After 24h of incubation, total RNA was isolated according to the standard method. |
Reaction Conditions | R2, 4µg/mL; 24h |
Applications | Rosmarinic Acid Promote NHE1 Expression In Vitro. |
| Animal experiment [2]: | |
Animal models | hyperlipidemia model |
Preparation Method | Male C57BL/6 mice (16–20g, 3 weeks old),Food intake and body weight were checked twice a week, and blood glucose concentrations were evaluated monthly in samples obtained from tails using a blood glucose meter. Mice with a fasting blood glucose (FBG) level ≥150mg/dl were considered hyperglycemic. Once hyperglycemia was achieved, the mice were randomly assigned to the following working groups: (1) the control group; the control group was fed a ND, (2) HFD group, (3) HFD + 50mg/kg rosmarinic acid group, (4) HFD + 100mg/kg rosmarinic acid group, and (5) HFD + 100mg/kg metformin group. Rosmarinic acid and metformin hydrochloride were dissolved in distilled water and administered via gastric gavage once a day for 8 weeks. Once treatment was complete, the mice were fasted overnight, FBG levels were measured, and an oral glucose tolerance test (OGTT) was performed. |
Dosage form | 50, 100mg/kg; po, 8 weeks |
Applications | Rosmarinic acid mediates reverse cholesterol transport through increased plasma cholesterol uptake and subsequent hepatobiliary excretion and induces lipid metabolism by increasing fatty acid β-oxidation in AMPK-dependent activation of CPT1A, thus modulating lipid accumulation and attenuating hyperlipidemia in HFD-fed mice. |
References: | |
| Cas No. | 20283-92-5 | SDF | |
| Chemical Name | (2R)-3-(3,4-dihydroxyphenyl)-2-[(E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl]oxypropanoic acid | ||
| Canonical SMILES | C1=CC(=C(C=C1CC(C(=O)O)OC(=O)C=CC2=CC(=C(C=C2)O)O)O)O | ||
| Formula | C18H16O8 | M.Wt | 360.33 |
| Solubility | ≥ 100.8 mg/mL in DMSO, ≥ 111.2 mg/mL in EtOH, ≥ 22.84 mg/mL in Water with gentle warming | Storage | Store at 2-8°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.7752 mL | 13.8762 mL | 27.7523 mL |
| 5 mM | 0.555 mL | 2.7752 mL | 5.5505 mL |
| 10 mM | 0.2775 mL | 1.3876 mL | 2.7752 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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