Rotenone (Synonyms: Nicouline, NSC 8505, NSC 26258, Tubatoxin) |
| Catalog No.GC16775 |
Rotenone is a mitochondrial electron transport chain complex I inhibitor that can induce apoptosis by enhancing the production of mitochondrial reactive oxygen species.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 83-79-4
Sample solution is provided at 25 µL, 10mM.
Rotenone is a mitochondrial electron transport chain complex I inhibitor that can induce apoptosis by enhancing the production of mitochondrial reactive oxygen species.
HL-60 cells were treated with rotenone (1 μ m ; 36 h) approximately 50% of HL-60 cells showed chromatin condensation and nuclear fragmentation under confocal microscopy[1].Rotenone(1μM/10 nM/100 nM;24-48h) causes dose-dependent cell death in SK-N-MC neuroblastoma cells[3].
Rotenone (2.5 mg/kg/day) for 4 weeks decreased the number of tyrosine hydroxylase (TH)-positive dopaminergic neurons in the SNpc and signal intensity of TH in the striatum[2]. In the renal ischemia-reperfusion model, Rotenone(200ppm;3weeks) can protect renal tubules, block renal fibrosis, reduce inflammation and apoptosis, and improve mitochondrial abnormalities[4].
References:
[1]. Li N, Ragheb K, Lawler G, Sturgis J, Rajwa B, Melendez JA, Robinson JP. Mitochondrial complex I inhibitor rotenone induces apoptosis through enhancing mitochondrial reactive oxygen species production. J Biol Chem. 2003 Mar 7;278(10):8516-25.
[2]. Miyazaki I, Isooka N, Imafuku F, Sun J, Kikuoka R, Furukawa C, Asanuma M. Chronic Systemic Exposure to Low-Dose Rotenone Induced Central and Peripheral Neuropathology and Motor Deficits in Mice: Reproducible Animal Model of Parkinson's Disease. Int J Mol Sci. 2020 May 4;21(9):3254.
[3] Sherer TB, Betarbet R, Testa CM, Seo BB, Richardson JR, Kim JH, Miller GW, Yagi T, Matsuno-Yagi A, Greenamyre JT. Mechanism of toxicity in rotenone models of Parkinson's disease. J Neurosci. 2003 Nov 26;23(34):10756-64.
[4] Zhang W, Sha Y, Wei K, Wu C, Ding D, Yang Y, Zhu C, Zhang Y, Ding G, Zhang A, Jia Z, Huang S. Rotenone ameliorates chronic renal injury caused by acute ischemia/reperfusion. Oncotarget. 2018 May 11;9(36):24199-24208.
| Cell experiment [1]: | |
Cell lines | HL-60 cells |
Preparation Method | HL-60 cells were treated with rotenone(1 μM ) for 36h. |
Reaction Conditions | Rotenone :1 μM ;36 h |
Applications | HL-60 cells were treated with rotenone (1 μM ; 36 h) approximately 50% of HL-60 cells showed chromatin condensation and nuclear fragmentation under confocal microscopy. |
| Animal experiment [2]: | |
Animal models | Pakanmori(PD) modle |
Preparation Method | Male C57BL/6J mice were injected subcutaneously with rotenone (2.5 mg/kg/day) for 4 weeks using an osmotic mini pump. |
Dosage form | Rotenone(2.5 mg/kg/day);4 weeks;sc |
Applications | Chronic subcutaneous treatment with a low dose of rotenone (2.5 mg/kg/day) for 4 weeks decreased the number of tyrosine hydroxylase (TH)-positive dopaminergic neurons in the SNpc and signal intensity of TH in the striatum. |
References: [1]. Li N, Ragheb K, Lawler G, Sturgis J, Rajwa B, Melendez JA, Robinson JP. Mitochondrial complex I inhibitor rotenone induces apoptosis through enhancing mitochondrial reactive oxygen species production. J Biol Chem. 2003 Mar 7;278(10):8516-25. [2]. Miyazaki I, Isooka N, Imafuku F, Sun J, Kikuoka R, Furukawa C, Asanuma M. Chronic Systemic Exposure to Low-Dose Rotenone Induced Central and Peripheral Neuropathology and Motor Deficits in Mice: Reproducible Animal Model of Parkinson's Disease. Int J Mol Sci. 2020 May 4;21(9):3254. | |
| Cas No. | 83-79-4 | SDF | |
| Synonyms | Nicouline, NSC 8505, NSC 26258, Tubatoxin | ||
| Chemical Name | (2R,6aR,12aS)-8,9-dimethoxy-2-(prop-1-en-2-yl)-1,2,12,12a-tetrahydrochromeno[3,4-b]furo[2,3-h]chromen-6(6aH)-one | ||
| Canonical SMILES | O=C1[C@H](C2=CC(OC)=C3OC)[C@@H](COC2=C3)OC4=C1C=CC5=C4C[C@H](C(C)=C)O5 | ||
| Formula | C23H22O6 | M.Wt | 394.42 |
| Solubility | ≥ 77.6mg/mL in DMSO | Storage | Store at 4°C, stored under nitrogen |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.5354 mL | 12.6768 mL | 25.3537 mL |
| 5 mM | 0.5071 mL | 2.5354 mL | 5.0707 mL |
| 10 mM | 0.2535 mL | 1.2677 mL | 2.5354 mL |
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Quality Control & SDS
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- Purity: >99.50%
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Average Rating: 5 (Based on Reviews and 38 reference(s) in Google Scholar.)
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