SDH-IN-2

SDH-IN-2 is a potent succinate dehydrogenase (SDH) inhibitor with an IC50 of 0.55 μg/mL. SDH-IN-2 is also an antifungal agent. SDH-IN-2 inhibits phytopathogenic fungia with average EC50 values of 3.82-9.81 μg/mL for all the fungi. SDH-IN-2 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

SDH-IN-2 (Compound 13) (200 μg/mL, 7 days) completely inhibits Physalospora piricola infection on apples, and also shows high inhibition rates of 70.9-86.9% on all the fungi even at 20 μg/mL^[1].
SDH-IN-2 inhibits P. oryza and F. oxysporum f. sp. niveum with EC₅₀s of 4.33 and 3.56 μg/mL^[1].
SDH-IN-2 (0-50 μg/mL) has no effect on the germination rate of bean seeds^[1].
SDH-IN-2 (0-100 μg/mL) has no significant effect on the growth of wheat seedlings^[1].
SDH-IN-2 inhibits SDH enzymatic activity with an IC₅₀ of 0.55 μg/mL^[1].
SDH-IN-2 binds well to the ubiquinone-binding region of SDH by hydrogen bonds and undergoes π–alkyl interaction and π-cation interaction^[1].

SDH-IN-2 (Compound 13) (0-200 μg/mL, 7 days) inhibits the infection of P. piricola on apples^[1].

References:
[1]. Zhang YH, et al. Discovery of N-Phenylpropiolamide as a Novel Succinate Dehydrogenase Inhibitor Scaffold with Broad-Spectrum Antifungal Activity on Phytopathogenic Fungi. J Agric Food Chem. 2023 Mar 1;71(8):3681-3693.