Protease and Phosphatase Inhibitor Cocktail (EDTA plus, 100X in ddH2O)

This product is a ready-to-use mixture of protease and phosphatase inhibitors (EDTA plus, 100X in ddH2O), which can effectively inhibit the activity of proteases and phosphatases in routine cell lysis and protein extraction. This product is suitable for experiments such as protein extraction of mammalian cells, primary cells, yeast cells, bacteria, animal tissues and plant tissues.
The main ingredients of this product include: serine|cysteine protease inhibitor Leupeptin, bovine trypsin inhibitor Aprotinin, aminopeptidase inhibitor Bestatin, cysteine protease inhibitor E-64, serine|threonine phosphatase reversible inhibitor β-glycerophosphate, serine|threonine phosphatase irreversible inhibitor Sodium Pyrophosphate, acid phosphatase inhibitor Sodium Fluoride, alkaline phosphatase|tyrosine phosphatase inhibitor Sodium Orthovanadate, and a metalloproteinase inhibitor EDTA aqueous solution is also provided.
This product can effectively inhibit the function of proteases such as serine proteases, leucine aminopeptidases, cysteine proteases, metalloproteases and aminopeptidase B, as well as phosphatases such as serine|threonine phosphatases, protein tyrosine phosphatases, acid phosphatases and alkaline phosphatases.
Recommended use: When lysing cells or extracting proteins, mix this product and lysis buffer at a volume ratio of 1:100.
EDTA in the product components may interfere with IMAC (immobilized metal affinity chromatography), protein stability (such as metal-containing proteins) or subsequent determinations. If you need to purify metalloproteases, aspartic acid proteases and Poly-His-tagged fusion proteins, it is recommended to use an EDTA-free protease and phosphatase inhibitor cocktail (GK10015).