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13(S)-HODE-d4

Catalog No.GC46420

An internal standard for the quantification of 13-HODE

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13(S)-HODE-d4 Chemical Structure

Cas No.: 139408-39-2

Tamaño Precio Disponibilidad Cantidad
25 μg
188,00 $
Disponible
50 μg
358,00 $
Disponible
100 μg
679,00 $
Disponible

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Sample solution is provided at 25 µL, 10mM.

Description of 13(S)-HODE-d4

13(S)-HODE-d4 contains four deuterium atoms at the 9, 10, 12, and 13 positions. It is intended for use as an internal standard for the quantification of 13(S)-HODE by GC- or LC-mass spectrometry. 13(S)-HODE is produced by incubation of linoleic acid with plant and mammalian lipoxygenases. It has been shown to inhibit the adhesion of tumor cells to the endothelium at concentrations around 1 µM,1,2 and down regulates the expression of the IRGpIIb/IIIa receptor.3

1.Buchanan, M.R., Haas, T.A., Lagarde, M., et al.13-Hydroxyoctadecadienoic acid is the vessel wall chemorepellant factor, LOXThe Journal of Biological Chemisty26016056-16059(1985) 2.Honn, K.V., Nelson, K.K., Renaud, C., et al.Fatty acid modulation of tumor cell adhesion to microvessel endothelium and experimental metastasisProstaglandins44(5)413-429(1992) 3.Grossi, I.M., Fitzgerald, L.A., Umbarger, L.A., et al.Bidirectional control of membrane expression and/or activation of the tumor cell IRGpIIb/IIIa receptor and tumor cell adhesion by lipoxygenase products of arachidonic acid and linoleic acidCancer Res.49(4)1029-1037(1989)

Chemical Properties of 13(S)-HODE-d4

Cas No. 139408-39-2 SDF
Canonical SMILES CCCCC[C@@](O)([2H])/C([2H])=C/C([2H])=C([2H])\CCCCCCCC(O)=O
Formula C18H28D4O3 M.Wt 300.5
Solubility DMF: >50 mg/ml (per Rao Maddipati),DMSO: >50 mg/ml (per Rao Maddipati),Ethanol: >50 mg/ml (per Rao Maddipati),PBS pH 7.2: >1 mg/ml (from 13(S)-HODE) Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of 13(S)-HODE-d4

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 3.3278 mL 16.6389 mL 33.2779 mL
5 mM 0.6656 mL 3.3278 mL 6.6556 mL
10 mM 0.3328 mL 1.6639 mL 3.3278 mL
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In vivo Formulation Calculator (Clear solution) of 13(S)-HODE-d4

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

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Average Rating: 5 ★★★★★ (Based on Reviews and 2 reference(s) in Google Scholar.)

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