Chicago Sky Blue 6B (Synonyms: Direct Blue 1) |
| Catalog No.GC10130 |
Chicago Sky Blue 6B is a counterstain for background autofluorescence in fluorescence and immunofluorescence histochemistry. Chicago Sky Blue 6B is a potent competitive inhibitor of vesicular glutamate transporter 1 (VGLUT1) with an IC50 of 3μM.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2610/5/1
Sample solution is provided at 25 µL, 10mM.
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Chicago Sky Blue 6B is a counterstain for background autofluorescence in fluorescence and immunofluorescence histochemistry[1]. Chicago Sky Blue 6B is a potent competitive inhibitor of vesicular glutamate transporter 1 (VGLUT1) with an IC50 of 3μM[2]. Chicago Sky Blue 6B is the first small molecule PrP ligand that can inhibit Aβ binding[3]. Chicago Sky Blue 6B has anti-inflammatory activity[4].
In vitro, Chicago Sky Blue 6B (3.3-10μM) treatment of Aβ- and lipopolysaccharide-treated BV2 microglia for 1h significantly inhibited the production of proinflammatory cytokines such as Tumor necrosis factor-α (TNF-α) and Interleukin-1β (IL-1β)[5]. Treatment of primary mouse bone marrow monocytes/macrophages (BMMs) with Chicago Sky Blue 6B (5, 10, 20μM) for 5-7 days inhibited osteoclast differentiation and bone resorption function and suppressed early NF-κB signaling[6].
In vivo, treatment of A53T α-synuclein transgenic (Tg) mice with Chicago Sky Blue 6B (20mg/kg/day) for 3 months rescued the motor coordination defects of Tg mice and significantly reduced the levels of S129-phosphorylated α-synuclein (pS129) in the brain regions and glial acidic protein (GFAP) immunoreactivity in the prefrontal cortex of Tg mice[7].
References:
[1] Priya S, Kaore N M M, Kaore S P N, et al. Utility of Chicago Sky Blue Stain in Rapid Diagnosis of Mycotic Infections[J]. National Journal of Laboratory Medicine, 2021.
[2] Favre-Besse F C, Poirel O, Bersot T, et al. Design, synthesis and biological evaluation of small-azo-dyes as potent Vesicular Glutamate Transporters inhibitors[J]. European Journal of Medicinal Chemistry, 2014, 78: 236-247.
[3] Risse E, Nicoll A J, Taylor W A, et al. Identification of a compound that disrupts binding of amyloid-β to the prion protein using a novel fluorescence-based assay[J]. Journal of Biological Chemistry, 2015, 290(27): 17020-17028.
[4] Pomierny B, Krzyżanowska W, Skórkowska A, et al. Inhibition of vesicular glutamate transporters (VGLUTs) with Chicago Sky Blue 6B before focal cerebral ischemia offers neuroprotection[J]. Molecular neurobiology, 2023, 60(6): 3130-3146.
[5] Yan S, Xuan Z, Yang M, et al. CSB6B prevents β-amyloid-associated neuroinflammation and cognitive impairments via inhibiting NF-κB and NLRP3 in microglia cells[J]. International Immunopharmacology, 2020, 81: 106263.
[6] Jin K, Zheng L, Ye L, et al. Chicago sky blue 6B (CSB6B), an allosteric inhibitor of macrophage migration inhibitory factor (MIF), suppresses osteoclastogenesis and promotes osteogenesis through the inhibition of the NF-κB signaling pathway[J]. Biochemical Pharmacology, 2021, 192: 114734.
[7] Min J O, Strohäker T, Jeong B C, et al. Chicago sky blue 6B inhibits α-synuclein aggregation and propagation[J]. Molecular brain, 2022, 15(1): 27.
| Cell experiment [1]: | |
Cell lines | BV2 cells |
Preparation Method | BV2 cells were pretreated with 1.1-10μM Chicago Sky Blue 6B as indicated for 1h, then treated with Aβ oligomer+LPS for 3h to test mRNA expression levels of IL-1β and TNF-α with RT-PCR. |
Reaction Conditions | 1.1-10μM; 1h |
Applications | Chicago Sky Blue 6B could significantly prevent Aβ oligomer and LPS combination-induced increase of IL-1β and TNF-α mRNA expression in BV2 cells. |
| Animal experiment [2]: | |
Animal models | A53T α-synuclein transgenic (Tg) mice |
Preparation Method | Chicago Sky Blue 6B (20mg/kg/day) or vehicle (PBS) was intraperitoneally injected into 6-month-old A53T α-synuclein transgenic (Tg) mice and wild-type (WT) littermates for 3 months. For behavioral tests, performed open field tests, balance beam tests, and forelimb grip strength tests. |
Dosage form | 20mg/kg/day for 3 months; i.p. |
Applications | Administration of Chicago Sky Blue 6B rescued the motor coordination deficits of Tg mice, significantly reduced the levels of S129-phosphorylated α-synuclein (pS129) in the brain regions of Tg mice and glial acidic protein (GFAP) immunoreactivity in the prefrontal cortex. |
References: | |
| Cas No. | 2610/5/1 | SDF | |
| Sinónimos | Direct Blue 1 | ||
| Chemical Name | sodium (6Z,6'Z)-6,6'-(2,2'-(3,3'-dimethoxy-[1,1'-biphenyl]-4,4'-diyl)bis(hydrazin-2-yl-1-ylidene))bis(4-amino-5-oxo-5,6-dihydronaphthalene-1,3-disulfonate) | ||
| Canonical SMILES | O=C1C(C(C=C/C1=N/NC(C(OC)=C2)=CC=C2C3=CC=C(C(OC)=C3)N/N=C4C(C(C(C=C\4)=C5S([O-])(=O)=O)=C(C(S([O-])(=O)=O)=C5)N)=O)=C6S([O-])(=O)=O)=C(C(S([O-])(=O)=O)=C6)N.[Na+].[Na+].[Na+].[Na+] | ||
| Formula | C34H24N6Na4O16S4 | M.Wt | 992.79 |
| Solubility | PBS (pH 7.2): 10 mg/ml | Storage | Store at 2-8°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.0073 mL | 5.0363 mL | 10.0726 mL |
| 5 mM | 0.2015 mL | 1.0073 mL | 2.0145 mL |
| 10 mM | 0.1007 mL | 0.5036 mL | 1.0073 mL |
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Quality Control & SDS
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- Purity: >95.00%
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Average Rating: 5 (Based on Reviews and 14 reference(s) in Google Scholar.)
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