Cy 3 (Non-Sulfonated) (triethylamine) (Synonyms: Cyanine3 triethylamine) |
Catalog No.GC66087 |
La trietilamina no sulfonada Cy 3 (Cyanine3) es un colorante de cianina (Cy) y un marcador fluorescente con un canal verde para proteÍnas y Ácidos nucleicos. La trietilamina no sulfonada Cy 3 es un fotoproducto fluorescente de Cyanine5 a través de la fotoconversiÓn tras la fotoexcitaciÓn. La trietilamina no sulfonada Cy 3 se puede utilizar para el seguimiento de partÍculas individuales de alta densidad en una célula viva sin utilizar iluminaciÓn ultravioleta ni aditivos tÓxicos para las células (Ex=470 nm; Em=515 nm y 565 nm nm).
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
Cy 3 Non-Sulfonated (Cyanine3) triethylamine is a cyanine (Cy) dye, and a fluorescent label with green channel for protein and nucleic acid. Cy 3 Non-Sulfonated triethylamine is a fluorescent photoproduct of Cyanine5 via photoconversion upon photoexcitation. Cy 3 Non-Sulfonated triethylamine can be used to high-density single-particle tracking in a living cell without using UV illumination and cell-toxic additives (Ex=470 nm; Em=515 nm and 565 nm nm)[1][2].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of Protein[2]:
1. Add protein (100 μL) to Cy 3 Non-Sulfonated triethylamine in 96 black micro-well for 50 min at 37 ??. Incubate the cells according to your normal protocol.
2. Scan plate using BioTek and collect data with excitation wavelength of 470 nm, and emission wavelength of 515 nm and 565 nm.
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