Dansylcadaverine (Monodansyl cadaverine) (Synonyms: Dansylcadaverine, MDC) |
| Catalog No.GC30072 |
Dansylcadaverine (Monodansyl cadaverine) (Monodansyl cadaverine) es un compuesto autofluorescente utilizado para el etiquetado de vacuolas autofÁgicas.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 10121-91-2
Sample solution is provided at 25 µL, 10mM.
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Dansylcadaverine is a fluorescent probe.
Detection of Dansylcadaverine fluorescence after SDS-PAGE shows that the amount of actin-thymosin β4 crosslink is very low in the presence of Dansylcadaverine and transglutaminase, while there are large amounts of Dansylcadaverine-labeled thymosin β4 and actin. The amount of cross-link further decreases in the presence of Dansylcadaverine competing for Lys residues of either G-actin or thymosin β4 (100 μM). In the absence of thymosin β4, Dansylcadaverine-labeled actin polymerized to F-actin as revealed by fluorescence detection after native PAGE[1].
[1]. Huff T, et al. Thymosin beta(4) serves as a glutaminyl substrate of transglutaminase. Labeling with fluorescentdansylcadaverine does not abolish interaction with G-actin. FEBS Lett. 1999 Dec 24;464(1-2):14-20. [2]. Laha D, et al. Interplay between autophagy and apoptosis mediated by copper oxide nanoparticles in human breast cancer cells MCF7. Biochim Biophys Acta. 2014 Jan;1840(1):1-9.
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Kinase experiment: |
To determine the time course of transglutamination, thymosin β4 (120 μM) is incubated with Dansylcadaverine (5 mM) in 70 μL buffer consisting of 10 mM Tris-HCl, pH 7.4, 15 mM CaCl2, 3 mM DTT. The reaction is started by addition of 0.1 U transglutaminase. Immediately after addition of the enzyme (t=0) and at indicated times,10 μL are taken from the mixture, diluted in 490 μL 0.1% TFA to stop the reaction and analyzed by HPLC[1]. |
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Cell experiment: |
MCF7 cells (2.4×104) are seeded into 35 mm plates. After 24 h incubation, CuO NPs are added with an increasing concentration in the presence or absence of 3-Methyladenine (3-MA) for different time periods . The cells are then incubated with 50 mM Dansylcadaverine (MDC) at 37°C for 15 min and washed with 1×PBS three times with 5 min interval. Finally, the cells are observed under a fluorescence microscope[2]. |
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References: [1]. Huff T, et al. Thymosin beta(4) serves as a glutaminyl substrate of transglutaminase. Labeling with fluorescentdansylcadaverine does not abolish interaction with G-actin. FEBS Lett. 1999 Dec 24;464(1-2):14-20. |
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| Cas No. | 10121-91-2 | SDF | |
| Sinónimos | Dansylcadaverine, MDC | ||
| Canonical SMILES | NCCCCCNS(=O)(C1=CC=CC2=C1C=CC=C2N(C)C)=O | ||
| Formula | C17H25N3O2S | M.Wt | 335.46 |
| Solubility | DMSO : 62.5 mg/mL (186.31 mM) | Storage | Store at -20°C, protect from light, stored under nitrogen |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.981 mL | 14.9049 mL | 29.8098 mL |
| 5 mM | 0.5962 mL | 2.981 mL | 5.962 mL |
| 10 mM | 0.2981 mL | 1.4905 mL | 2.981 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >97.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 23 reference(s) in Google Scholar.)
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