5-Hydroxymethylcytidine-5'-Triphosphate (Synonyms: 5-Hydroxymethyl-CTP) |
| Catalog No.GN20050 |
5-Hydroxymethylcytidine-5-Triphosphate es un citidina trifosfato modificado con un grupo hidroximetilo en la posición 5 del carbono. Puede ser utilizado para reemplazar el ARNm no modificado y mejorar la estabilidad y funcionalidad del ARN.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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5-Hydroxymethylcytidine-5-Triphosphate es un citidina trifosfato modificado con un grupo hidroximetilo en la posición 5 del carbono. Puede ser utilizado para reemplazar el ARNm no modificado y mejorar la estabilidad y funcionalidad del ARN[1]. La función de modificación del ARNm de 5-Hidroximetilcitosina-5-Trifosfato tiene una amplia gama de aplicaciones en la investigación de expresión génica, el desarrollo de fármacos basados en ARN y la terapia génica[2]. 5-Hidroximetilcitosina-5-Trifosfato puede reducir la respuesta inmune innata en células humanas y de otros mamíferos[3, 4].
References:
[1] Fu L, Guerrero C R, Zhong N, et al. Tet-mediated formation of 5-hydroxymethylcytosine in RNA[J]. Journal of the American Chemical Society, 2014, 136(33): 11582-11585.
[2] Kuszczynska A, Bors M, Podskoczyj K, et al. Chemistry of installing epitranscriptomic 5-modified cytidines in RNA oligomers[J]. Organic & Biomolecular Chemistry, 2024, 22(36): 7271-7286.
[3] Lu M, Xue M, Wang H T, et al. Nonsegmented negative-sense RNA viruses utilize N 6-methyladenosine (m6A) as a common strategy to evade host innate immunity[J]. Journal of virology, 2021, 95(9): 10.1128/jvi. 01939-20.
[4] Cui L, Ma R, Cai J, et al. RNA modifications: importance in immune cell biology and related diseases[J]. Signal transduction and targeted therapy, 2022, 7(1): 334.
| Cas No. | SDF | ||
| Sinónimos | 5-Hydroxymethyl-CTP | ||
| Formula | C10H18N3O15P3 (free acid) | M.Wt | 513.10g/mole (free) |
| Solubility | Storage | -20°C or below | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 35 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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