Janelia Fluor 549, free acid (Synonyms: JF549 TFA) |
| Catalog No.GC50479 |
Janelia Fluor 549, Ácido libre (JF549 TFA) es un tinte fluorescente con un mÁximo de absorciÓn (Λab (max)) de 549 nm y un mÁximo de emisiÓn (Λem (max)) de 571 nm.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2245946-45-4
Sample solution is provided at 25 µL, 10mM.
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Yellow fluorescent dye; supplied as a free acid. Used in protocol (2017 Grimm et al - see references below) for the synthesis of Janelia Fluor® HaloTag® and SNAP-Tag® ligands, for use in live cell imaging experiments. Also suitable for flow cytometry. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1.
Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat. Methods 12 244 PMID:25599551 |Grimm et al (2017) Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments. Methods Mol.Biol. 1663 179 PMID:28924668
| Cas No. | 2245946-45-4 | SDF | |
| Sinónimos | JF549 TFA | ||
| Canonical SMILES | OC(C1=CC=C(C(C(C2=CC=C(C=C2OC3=C/4)N5CCC5)=C3C=CC4=[N+]6CCC/6)=C1)C([O-])=O)=O.FC(F)(F)C(O)=O | ||
| Formula | C27H22N2O5.C2HF3O2 | M.Wt | 568.5 |
| Solubility | DMSO : 25 mg/mL (43.98 mM; Need ultrasonic) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.759 mL | 8.7951 mL | 17.5901 mL |
| 5 mM | 0.3518 mL | 1.759 mL | 3.518 mL |
| 10 mM | 0.1759 mL | 0.8795 mL | 1.759 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 20 reference(s) in Google Scholar.)
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