L-a-Hydroxyglutaric acid disodium salt (Synonyms: L-2-HG, L-2-Hydroxyglutarate, L-2-Hydroxyglutaric Acid, (S)-2-Hydroxypentanedioic Acid) |
| Catalog No.GC11149 |
La sal disÓdica del Ácido L-a-hidroxiglutÁrico es un modificador epigenético y un oncometabolito putativo en el cÁncer renal. La sal disÓdica del Ácido L-a-hidroxiglutÁrico puede inhibir las histonas desmetilasas y, por lo tanto, promover la metilaciÓn de las histonas. El Ácido L-2-hidroxiglutÁrico inhibe la actividad de la creatina quinasa mitocondrial (Mi-CK) con Km y Ki de 2,52 mM y 11,13 mM, respectivamente.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 63512-50-5
Sample solution is provided at 25 µL, 10mM.
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IC50: N/A
L-α-Hydroxyglutaric acid disodium salt is a salt form of L-α-Hydroxyglutaric acid. L-α-Hydroxyglutaric acid accumulates as a result of a rare defect in L-2-HG dehydogenase, resulting in the metabolic disorder L-α-hydroxyglutaric aciduria.
In vitro: It has been reported that L-α-Hydroxyglutaric acid disodium salt could be used as the collection buffer for increasing the recovery of hypoxia-inducible factor-1 α, which was a marker of hypoxia in human tumors [1]. In another previous study, L-α-Hydroxyglutaric acid disodium salt was also used in the radiolabeled conversion assay to evaluate its effect on the TET family of methyl hydroxylases [2].
In vivo: In the 30-day-old rats, L-α-Hydroxyglutaric acid was found to be able to significantly increase chemiluminescence, PCF and TBA-RS measurements and decrease the TAR values in cerebellum markedly. In addition, the L-α-Hydroxyglutaric acid-induced increase of TBA-RS was significantly attenuated by melatonin and also by the combinations of ascorbic acid and soluble alpha-tocopherol (Trolox) and of superoxide dismutase plus catalase but not by the inhibitor of nitric oxide synthase Nomega-nitro-L-arginine methyl ester (L-NAME), , superoxide dismutase or creatine or catalase alone in either cerebral structure [3].
Clinical trial: Up to now, L-a-Hydroxyglutaric acid disodium salt is still in the preclinical development stage.
References:
[1] Park SR,Kinders RJ,Khin S,et al. Validation of a hypoxia-inducible factor-1 alpha specimen collection procedure and quantitative enzyme-linked immunosorbent assay in solid tumor tissues. Anal Biochem.2014 Aug 15;459:1-11.
[2] Evans B,Griner E. Registered report: Oncometabolite 2-hydroxyglutarate is a competitive inhibitor of α-ketoglutarate-dependent dioxygenases. Elife.2015 Jul 31;4:e07420.
[3] Latini A,Scussiato K,Rosa RB,Leipnitz G,Llesuy S,Belló-Klein A,Dutra-Filho CS,Wajner M. Induction of oxidative stress by L-2-hydroxyglutaric acid in rat brain. J Neurosci Res.2003 Oct 1;74(1):103-10.
| Cas No. | 63512-50-5 | SDF | |
| Sinónimos | L-2-HG, L-2-Hydroxyglutarate, L-2-Hydroxyglutaric Acid, (S)-2-Hydroxypentanedioic Acid | ||
| Chemical Name | sodium (S)-2-hydroxypentanedioate | ||
| Canonical SMILES | O=C([O-])[C@@H](O)CCC([O-])=O.[Na+].[Na+] | ||
| Formula | C5H6Na2O5 | M.Wt | 192.08 |
| Solubility | ≥ 19.2mg/mL in Water | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.2062 mL | 26.0308 mL | 52.0616 mL |
| 5 mM | 1.0412 mL | 5.2062 mL | 10.4123 mL |
| 10 mM | 0.5206 mL | 2.6031 mL | 5.2062 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 9 reference(s) in Google Scholar.)
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