Retinol (Vitamin A1) (Synonyms: all-trans Retinol, all-trans Vitamin A, NSC 122759, Vitamin A1 Alcohol) |
| Catalog No.GC31345 |
Retinol, also known as vitamin A1, is a fat-soluble vitamin in the vitamin A family that is found in food and used as a dietary supplement[1].
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 68-26-8
Sample solution is provided at 25 µL, 10mM.
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Retinol, also known as vitamin A1, is a fat-soluble vitamin in the vitamin A family that is found in food and used as a dietary supplement[1]. Retinol is typically measured in International Units (IU), with 1 IU of retinol equivalent to about 0.3 micrograms[2]. Retinol is transported in plasma, bound to specific transport proteins known as retinol-binding proteins (RBP)[3]. Also known as all-trans retinol, it primarily exerts its effects by activating retinoic acid receptors (RARs) and retinoid X receptors (RXRs), which are part of the nuclear receptor family, regulating gene expression and thereby affecting cell differentiation, proliferation, and death[4].
In vitro, retinol at concentrations of 0.1-3.5µM was added to human embryonic stem cell (hESC) culture media and continuously passaged. 2.0 µM of retinol promoted the proliferation of human embryonic stem cells and induced the expression of specific markers, indicating that retinol has the function of supporting stem cell self-renewal[5]. Retinol increased the expression of the NANOG gene, with its relative expression level increasing by 20 times in the presence of 2.0-3.5 µM retinol[5]. Additionally, retinol maintained the growth of undifferentiated cancer-like stem cells from mouse mammary tumors in long-term culture while eliminating non-CSCs[6]. In addition, 0.1% retinol can effectively treat skin photodamage, promote keratinocyte proliferation and epidermal thickening, thereby alleviating signs of skin aging[7].
References:
Low, J W, Arimond, et al.A food-based approach introducing orange-fleshed sweet potatoes increased vitamin A intake and serum retinol concentrations in young children in rural Mozambique.[J].Journal of Nutrition, 2007.
Shenai J .Vitamin A Supplementation in Very Low Birth Weight Neonates: Rationale and Evidence[J]. 2000.
[3] DeWitt,S,Goodman.PLASMA RETINOL-BINDING PROTEIN[J].Annals of the New York Academy of Sciences, 1980.
[4] Mangelsdorf D J , Umesono K , Kliewer S A ,et al.A direct repeat in the cellular retinol-binding protein type II gene confers differential regulation by RXR and RAR.[J].Cell, 1991, 66(3):555-61.
[5] Rajala K , Vaajasaari H , Suuronen R ,et al.Effects of the physiochemical culture environment on the stemness and pluripotency of human embryonic stem cells[J].Stem Cell Studies, 2011.
[6]Khillan J S .Vitamin A/Retinol and Maintenance of Pluripotency of Stem Cells[J]. Multidisciplinary Digital Publishing Institute, 2014.
[7]Tucker-Samaras S , Zedayko T , Cole C , et al. A stabilized 0.1% retinol facial moisturizer improves the appearance of photodamaged skin in an eight-week, double-blind, vehicle-controlled study[J]. Journal of Drugs in Dermatology, 2009, 8(10):932-936.
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Cell experiment [1]: |
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Cell lines |
hESC cell lines |
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Preparation method |
Plate at a density of 3.8×104 cells/cm2. Add 0.5, 2.0, and 3.5 μM Retinol and lipid derivatives respectively, monitor the growth of hESCs using a microscope, and change the culture medium six times a week. hESCs were mechanically passaged into new mitotically inactive feeder cells every 6 to 7 days. |
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Reaction Conditions |
0.5, 2.0, and 3.5 μM；multiple days |
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Applications |
Low concentrations such as 0.1–0.5μM Retinol had no effect on the self-renewal of human ESCs whereas 2.0μM Retinol enhanced their proliferation and induced the expression of human ESC specific markers. |
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References: [1] Rajala K , Vaajasaari H , Suuronen R ,et al.Effects of the physiochemical culture environment on the stemness and pluripotency of human embryonic stem cells[J].Stem Cell Studies, 2011. |
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| Cas No. | 68-26-8 | SDF | |
| Sinónimos | all-trans Retinol, all-trans Vitamin A, NSC 122759, Vitamin A1 Alcohol | ||
| Canonical SMILES | CC(/C=C/C=C(/C=C/C1=C(CCCC(C)1C)C)C)=C\CO | ||
| Formula | C20H30O | M.Wt | 286.45 |
| Solubility | Chloroform: 10 mg/ml;DMF: 30 mg/ml;DMSO: 30 mg/ml;Ethanol: 10 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.491 mL | 17.4551 mL | 34.9101 mL |
| 5 mM | 0.6982 mL | 3.491 mL | 6.982 mL |
| 10 mM | 0.3491 mL | 1.7455 mL | 3.491 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 35 reference(s) in Google Scholar.)
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