Sphingosine-1-phosphate (Synonyms: Sphingosine-1-phosphate (d18:1); S1P (d18:1); Sphingosine-1-Phosphoric Acid) |
| Catalog No.GC17004 |
Sphingosine 1-phosphate is a metabolic product of cell membrane sphingolipids.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 26993-30-6
Sample solution is provided at 25 µL, 10mM.
Sphingosine 1-phosphate is a metabolic product of cell membrane sphingolipids. It is bound to extracellular chaperones, enriched in circulatory fluids and binds to G protein-coupled Sphingosine 1-phosphate receptors (Sphingosine 1-phosphateRs) used for regulating embryonic development, postnatal organ function and disease.[1]
In vitro, treatment with 200 and 400 μM Sphingosine 1-phosphate in human ovarian cortical samples in dose-dependent decrease in the protein expression of cleaved caspase-3 using western blot and in the number of apoptotic follicles stained positive for cleaved caspase-3 using immunohistochemistry.[2] In vitro, with 0.1-10 μM Sphingosine 1-phosphate in a concentration-dependent manner evoked action potential (AP) generation by single fibre electrophysiological recordings.[3] In addition, using Ca2+ imaging experiments, Sphingosine 1-phosphate at 1 μM can elicit a transient increase in intracellular Ca2+ in astrocytes, followed by sustained elevation.[4]
In vivo, superfusion of 0.001-10 μM Sphingosine 1-phosphate evoked in concentration-dependent manner vasoconstriction in preglomerular microvessels, predominantly afferent arterioles. [5] In vivo experiment it shown that injection 10 mg/kg sphingosine 1-phosphate intravenously in mice caused immediate rigor and death. [6] In male beagles, treatment with 85 ug/kg Sphingosine 1-phosphate intravenously decreased the formation of Q(s)/Q(t) (32%), and both the presence of protein (72%) and neutrophils (95%) in BAL fluid compared with vehicle controls. However, Sphingosine 1-phosphate increased the LPS-induced systemic production of three inflammatory cytokines, TNF-alpha (6-fold), KC (1.2-fold), and IL-6 (3-fold), without resulting in end-organ dysfunction.[7]
References:
[1]Cartier A, et al. Sphingosine 1-phosphate: Lipid signaling in pathology and therapy. Science. 2019 Oct 18;366(6463):eaar5551.
[2]Guzel Y, et al. Sphingosine-1-phosphate protects human ovarian follicles from apoptosis in vitro. Eur J Obstet Gynecol Reprod Biol. 2018 Mar;222:19-24.
[3]Patil MJ, et al. Sphingosine-1-phosphate activates mouse vagal airway afferent C-fibres via Sphingosine 1-phosphateR3 receptors. J Physiol. 2019 Apr;597(7):2007-2019.
[4]Shirakawa H, et al. Sphingosine-1-phosphate induces Ca2+ signaling and CXCL1 release via TRPC6 channel in astrocytes. Glia. 2017 Jun;65(6):1005-1016.
[5]Guan Z, et al. Sphingosine-1-phosphate evokes unique segment-specific vasoconstriction of the renal microvasculature. J Am Soc Nephrol. 2014 Aug;25(8):1774-85.
[6]Igarashi Y, et al. Sphingosine 1-phosphate is a blood constituent released from activated platelets, possibly playing a variety of physiological and pathophysiological roles. Acta Biochim Pol. 1998;45(2):299-309.
[7]Szczepaniak WS,et al. Sphingosine 1-phosphate rescues canine LPS-induced acute lung injury and alters systemic inflammatory cytokine production in vivo. Transl Res. 2008 Nov;152(5):213-24.
| Cas No. | 26993-30-6 | SDF | |
| Synonyms | Sphingosine-1-phosphate (d18:1); S1P (d18:1); Sphingosine-1-Phosphoric Acid | ||
| Chemical Name | (2S,3R,E)-2-amino-3-hydroxyoctadec-4-en-1-yl dihydrogen phosphate | ||
| Canonical SMILES | O[C@H](/C=C/CCCCCCCCCCCCC)[C@H](COP(O)(O)=O)N | ||
| Formula | C18H38NO5P | M.Wt | 379.5 |
| Solubility | ≤4 mg/ml in 0.3M NaOH | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.635 mL | 13.1752 mL | 26.3505 mL |
| 5 mM | 0.527 mL | 2.635 mL | 5.2701 mL |
| 10 mM | 0.2635 mL | 1.3175 mL | 2.635 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Related Biological Data
Product ion mass spectra of sphingosine (d16:0). (D) sphingosine-1-phosphate
The storage solution of S1P (GlpBio, USA) was confgured by (DMSO/concentrated hydrochloric acid (100:2), v/v) at a concentration of 500μg/mL.
Lipids Health Dis 22.1 (2023): 1-15. PMID: 36698123 IF: 4.3212 -
Related Biological Data
Vitamin D increases cell-substrate attachment quality by stabilizing F-actin in PC12 cells. (C) PC12 cells were treated with or without 2uM S1P and 100nM 1,25- (OH)2D3 for 48h respectively, F-actin was stained by fluorescein analysised by EVOS M7000 3D digital confocal fluorescence microscopy.
PC12 cells were treated with 2uM S1P (GlpBio, USA) and 100nM 1,25-(OH)2D3 for 48h respectively, stained with 4.5uM PI(red, stained cells) for 30min in 37 ℃ incubator avoiding light.
J Steroid Biochem, 2024, 236: 106425. PMID: 37984747 IF: 4.0998
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