SuperFast RT Master Mix for qPCR (gDNA remover)

SuperFast RT Master Mix for qPCR (gDNA remover) is a reverse transcription kit with ultra-high sensitivity. It consists of a reverse transcription reaction premix and a gDNA removal mix, and also provides a portion of DEPC-ddH2O for dilution. Obtaining cDNA with SuperFast RT Master Mix for qPCR (gDNA remover) is the first step of a two-step RT-PCR experiment. The obtained cDNA can be used for conventional PCR or RT-PCR. If you are doing an RT-PCR experiment, it is recommended to use it with SYBR Green qPCR Master Mix (Catalog Number: GK10002).

SuperFast RT Master Mix for qPCR (gDNA remover) uses the high temperature (>50℃) high-speed amplification reverse transcriptase Super Reverse Transcriptase, which can synthesize cDNA from extremely low amounts (pg~μg level) of total RNA or poly(A) mRNA, and is compatible with RNA templates with high GC content and complex secondary structure.

In many cases, RNA extracted by methods such as spin columns or acid guanidine-phenol chloroform (AGPC) method will contain a small amount of genomic DNA (gDNA). When pseudogenes exist in the target gene or primers cannot be designed across introns, genomic DNA will be amplified together with cDNA, thus affecting the accuracy of the data. SuperFast RT Master Mix for qPCR (gDNA remover) contains a gDNA remover with strong DNA degradation ability, which helps to remove gDNA and reverse transcribe RNA without purification.

[Product Features]

1. Effective removal of gDNA: It only takes 2 minutes to remove genomic DNA contamination.

2. High efficiency and fast: This kit uses extremely fast amplification reverse transcriptase and optimized oligonucleotide dT primers to achieve efficient reverse transcription, and the reaction can be completed in just 5 minutes.

3. Ultra-high sensitivity: Good reverse transcription reaction can also be performed on very small amounts of RNA templates.

4. Super compatibility: It can be compatible with RNA templates with high GC content and complex secondary structure.

5. Ultra-low impact factors: Super strong applicability to subsequent qPCR reactions: Through continuous optimization, the impact of this kit on subsequent qPCR reactions is minimized.