TFAX 488, SE |
| Catalog No.GC50521 |
TFAX 488, SE is a green fluorescent dye and exhibits pH-insensitivity over a very broad range (pH in the 4-10).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 222164-96-7
Sample solution is provided at 25 µL, 10mM.
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Amine reactive green fluorescent dye. Insensitive to pH in the range 4 - 10. Forms bright and photostable conjugates with proteins and antibodies. Suitable for use in flow cytometry, two-photon excitation microscopy (TPE), and super resolution microscopy techniques, such as dSTORM, SIM and STED. Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1cm-1; quantum yield = 0.92.
Rossi et al (2012) Super-resolution imaging of aquaporin-4 orthogonal arrays of particles in cell membranes. J.Cell Sci. 125 4405 PMID:22718347 |Burnette et al (2011) Bleaching/blinking assisted localization microscopy for superresolution imaging using standard fluorescent molecules. Proc.Natl.Acad.Sci.USA. 108 21081 PMID:22167805 |van de Linde et al (2011) Direct stochastic optical reconstruction microscopy with standard fluorescent probes. Nat.Protoc. 6 991 PMID:21720313 |Iwata et al (2005) Increased susceptibility of cytoplasmic over nuclear polyglutamine aggregates to autophagic degradation. Proc.Natl.Acad.Sci.USA. 102 13135 PMID:16141322 |Panchuk-Voloshina et al (1999) Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J.Histochem.Cytochem. 47 1179 PMID:10449539
| Cas No. | 222164-96-7 | SDF | |
| Canonical SMILES | [NH2+]=C1C=CC2=C(C3=CC=C(C(ON4C(CCC4=O)=O)=O)C=C3C([O-])=O)C5=CC=C(N)C(S(=O)([O-])=O)=C5OC2=C1S(=O)([O-])=O | ||
| Formula | C25H15N3O13S2Li2 | M.Wt | 643.41 |
| Solubility | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.5542 mL | 7.7711 mL | 15.5422 mL |
| 5 mM | 0.3108 mL | 1.5542 mL | 3.1084 mL |
| 10 mM | 0.1554 mL | 0.7771 mL | 1.5542 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 11 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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