Vitamin B12 (hydrate) (Synonyms: Cobalamin) |
| Catalog No.GC48253 |
A neuropeptide with diverse biological activities
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 194937-59-2
Sample solution is provided at 25 µL, 10mM.
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Vitamin B12 is a water-soluble vitamin with roles in brain and nervous system functioning and blood formation through regulation of DNA synthesis, cellular metabolism, fatty acid metabolism, and amino acid metabolism.1 It belongs to the corrinoid group of compounds, which contain a corrin macrocycle, and is produced only by certain bacteria and archaea.2 Reference to vitamin B12 usually encompasses a group of cobalt-containing compounds (or cobalamins), which include cyanocobalamin, hydroxocobalamin, 5'-deoxyadenosylcobalamin (AdoB12), and methylcobalamin (MeB12). These forms serve as cofactors in isomerase-, methyltransferase-, and dehalogenase-catalyzed reactions.3 For example, methylmalonyl coenzyme A mutase acts on AdoB12 to convert methylmalonyl-CoA to succinyl-CoA, an important step before entry into the Kreb's cycle. Additionally, methionine synthase uses MeB12 to transfer a methyl group from 5-methyltetrahydrofolate to homocysteine to generate tetrahydrofolate and methionine, which is important for DNA synthesis.4
1.Medicine, I.o.Vitamin B12Dietary reference intakes for thiamin, riboflavin, niacin, vitamin B6, folate, vitamin B12, pantothenic acid, biotin, and choline306-356(1998) 2.Moore, S.J., Lawrence, A.D., Biedendieck, R., et al.Elucidation of the anaerobic pathway for the corrin component of cobalamin (vitamin B12)Proc. Natl. Acad. Sci. USA110(37)14906-14911(2015) 3.Banerjee, R., and Ragsdale, S.W.The many faces of vitamin B12: Catalysis by cobalamin-dependent enzymesAnnual Reviews of Biochemistry72209-247(2003) 4.Ragsdale, S.W.Catalysis of methyl group transfers involving tetrahydrofolate and B12Vitamins and Hormones79293-324(2008)
| Cas No. | 194937-59-2 | SDF | |
| Synonyms | Cobalamin | ||
| Canonical SMILES | C[C@@]12[N]([Co+3]34([N-]5[C@]2([H])[C@H](CC(N)=O)[C@@](C)(CCC(NC[C@@H](C)O[P]6([O-])=O)=O)C5=C(C)C([C@@H](CCC(N)=O)C7(C)C)=[N]3C7=CC8=[N]4C([C@](CC(N)=O)(C)[C@@H]8CCC(N)=O)=C9C)([N]%10=CN([C@]%11([H])[C@H](O)[C@H](O6)[C@@H](CO)O%11)C%12=CC(C)=C(C)C=C%10%12)[C]#N)=C9[C@@H](CCC(N)=O)[C@]1(C)CC(N)=O.O | ||
| Formula | C63H88CoN14O14P.XH2O | M.Wt | 1355.4 |
| Solubility | DMSO: 75 mg/ml,Ethanol: 10 mg/ml,Water: 50 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 0.7378 mL | 3.6889 mL | 7.3779 mL |
| 5 mM | 0.1476 mL | 0.7378 mL | 1.4756 mL |
| 10 mM | 0.0738 mL | 0.3689 mL | 0.7378 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 31 reference(s) in Google Scholar.)
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